亚博体育官网

亚博体育|即时比分|即时直播

登录    |    注册

您好,欢迎来到中国亚博体育资讯平台!

首页> 《中国测试》期刊 >本期导读>甘露饮中六种有效成分提取及含量测定

甘露饮中六种有效成分提取及含量测定

182    2019-06-26

¥0.00

全文售价

作者:黄亚1, 宿怀予1, 范秀2, 周荣1, 廖昌军2

作者单位:1. 四川省德阳市人民医院, 四川 德阳 618000;
2. 成都医学院药学院, 四川 成都 610083


关键词:甘露饮;正交试验;绿原酸;麦角甾苷;柚皮苷;黄芩苷;高效液相色谱


摘要:

探讨甘露饮中6种有效成分(绿原酸、麦角甾苷、柚皮苷、黄芩苷、甘草酸、熊果酸)最佳提取工艺条件及含量测定方法。优化提取溶剂,通过正交试验法考察料液比、提取时间和提取次数对甘露饮提取工艺的影响。采取Syncronis C18色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈(A)-0.1%磷酸水溶液(B)梯度洗脱(0~5 min,5% A;5~40 min,5%~20% A;40~60 min,20%~50% A;60~80 min,50%~90% A;80~90.1 min,90%~5% A;90.1~100 min,5% A),流量1.0 mL/min,柱温35 ℃,检测波长210 nm,进样量20 μL的色谱条件对甘露饮提取物中6种有效成分进行含量测定。最佳提取工艺条件是:60%乙醇为提取溶剂,料液比1∶4,提取时间为1 h,提取次数为1次。绿原酸、麦角甾苷、柚皮苷、黄芩苷、甘草酸、熊果酸分别在4.80~80.00,10.00~100.00,24.00~400.00,30.00~500.00,6.00~100.00,3.60~60.00 μg/mL浓度范围内线性关系良好(r2≥0.999 5)。平均加样回收率在96.12%~101.82%,RSD在1.72%~2.92%。该提取工艺稳定可行,HPLC法能简便、准确地测定甘露饮提取物中6种有效成分的含量。


Extraction and content determination of six effective components from Ganlu Yin
HUANG Ya1, SU Huaiyu1, FAN Xiu2, ZHOU Rong1, LIAO Changjun2
1. Deyang People's Hospital in Sichuan Province, Deyang 618000, China;
2. School of Pharmacy, Chengdu Medical College, Chengdu 610083, China
Abstract: To investigate the optimum extraction condition and content determination of six effective components (chlorogenic acid, acteoside, naringin, baicalin, glycyrrhizic acid, ursolic acid) in Ganlu Yin. Extraction solvent was optimized, the effects of the amount of solid-liquid ratio, extraction time and extraction times on the extraction technology of Ganlu Yin were investigated by orthogonal test. Six effective components in the Ganlu Yin extracts were determined on Syncronis C18 column (4.6 mm×250 mm, 5 μm), and eluted with the mobile phase of acetonitrile (A) and 0.1% phosphoric acid (B) with linear gradient mode (0-5 min, 5% A; 5-40 min, 5%-20% A; 40-60 min, 20%-50% A; 60-80 min, 50%-90% A; 80-90.1 min, 90%-5% A; 90.1-100 min, 5% A), the flow rate of the mobile phase was 1.0 mL/min with the column temperature of 35℃, and the detection wavelengths were at 210 nm,and the sample size of 20 μL. The optimum extraction process was as follows:60% ethanol as extraction solvent, solid-liquid ratio 1:4, 1h for each time and extracting one time. The linea ranges of chlorogenic acid, acteoside, naringin, baicalin, glycyrrhizic acid and ursolic acid was 4.80-80.00, 10.00-100.00, 24.00-400.00, 30.00-500.00, 6.00-100.00, 3.60-60.00 μg/mL (r2 ≥ 0.999 5), respectively. The mean recoveries were 96.12%-101.82% with RSD between 1.72%~2.92%.This extraction process is stable and feasible, and the six effective components in Ganlu Yin extracts can be determined simply and accurately by HPLC.
Keywords: Ganlu Yin;orthogonal test;chlorogenic acid;acteoside;naringin;baicalin;HPLC
2019, 45(6):81-87  收稿日期: 2018-11-12;收到修改稿日期: 2018-12-20
基金项目: 德阳市科技局支撑计划(2016SZ033)
作者简介: 黄亚(1988-),少女,四川成都市人,主管药师,硕士,主要从事临床药学研究
参考文献
[1] 宋. 太平惠民和剂局编. 太平惠民和剂局方[M]. 北京:人民卫生出版社, 1985:308.
[2] 路军章, 张蕾. 中医药防治急性放射性食管炎[J]. 中华中医药杂志, 2012, 27(12):3019-3022
[3] 罗素芳. 甘露饮加减在阴虚湿热证型角结膜干燥症治疗中的疗效观察[J]. 中医临床研究, 2017, 9(32):113-114
[4] 严永旺, 肖兰, 周旭, 等. 绿原酸的药理作用及药用研发对策[J]. 中国药房, 2017, 28(19):2729-2732
[5] 金元宝, 刘萍, 刘小根, 等. 柚皮苷的生物活性研究进展[J]. 中国现代医药杂志, 2018, 20(3):92-97
[6] XU L, LI J, ZHANG Y, et al. Regulatory effect of baicalin on the imbalance of Th17/Treg responses in mice with allergic asthma[J]. Journal of Ethnopharmacology, 2017, 208:199-206
[7] 周红潮, 杜锐, 王慧, 等. 黄芩苷药代动力学研究进展[J]. 中国中药杂志, 2018, 43(4):684-688
[8] 万嘉洋, 张宇燕, 黄平, 等. 甘草中甘草酸的粗提工艺研究[J]. 中华中医药学刊, 2017, 35(1):149-151
[9] 王韵, 赵亮, 柴逸峰, 等. RP-HPLC法同时测定茵陈蒿汤中14个成分[J]. 药物分析杂志, 2017, 37(3):393-401
[10] 林鹏. RP-HPLC法同时测定生地黄中麦角甾苷和马替诺皂苷的含量[J]. 医学影像与检验, 2017, 4(14):273
[11] 邓桂珠, 蓝文煜, 窦佩丹, 等. 枳壳及其炮制品色差值与化学成分的相关性[J]. 中成药, 2017, 39(6):1228-1231
[12] 于东平. 甘露饮合剂的制备与质量控制[J]. 中国中医药咨询, 2011, 3(15):375-376
[13] 夏雪, 王溪晓, 任国庆, 等. 26批不同来源枳壳中成分柚皮苷与橙皮苷及新橙皮苷含量分析[J]. 亚太传统医药, 2018, 14(6):58-60
[14] 刘慧妍, 黄馨慧, 张艳海. HPLC法快速测定8种中药中齐墩果酸和熊果酸[J]. 中草药, 2017, 48(10):1998-2001
[15] 周倩, 戴衍朋, 王亮, 等. HPLC法测定生甘草、炙甘草中6种成分[J]. 中成药, 2016, 38(2):378-382