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首页>《中国测试》期刊>本期导读>云南凤庆大叶种晒青茶茶多酚提取工艺优化及抗氧化研究

云南凤庆大叶种晒青茶茶多酚提取工艺优化及抗氧化研究

207    2019-05-28

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作者:段凤敏1, 张绍旺1, 邵维炯2, 高华磊2, 匡兴贵2, 饶杰1, 保志娟2

作者单位:1. 云南省计量测试技术研究院, 云南 昆明 650228;
2. 云南农业大学烟草学院, 云南 昆明 650201


关键词:大叶种;晒青毛茶;茶多酚;响应面分析;抗氧化活性


摘要:

以云南凤庆大叶种晒青毛茶为原料,在单因素实验的基础上,通过Box-Behnken实验设计及响应面分析法,研究乙醇浓度、液料比、提取温度、提取时间对晒青毛茶茶多酚提取率的影响,同时通过超氧阴离子、羟基自由基和DPPH自由基的清除能力评价晒青毛茶茶多酚的抗氧化性能。结果表明,0.2 g茶粉在固定超声波功率为160 W下,预测最佳提取工艺为:乙醇浓度60.01%,液料比49.28∶1 mL/g,超声时间25 min,超声温度30℃,总多酚得率为19.905%(验证值为19.83%)。云南大叶种晒青毛茶茶多酚具有很强的抗氧化能力,对超氧阴离子、羟基自由基、DPPH自由基清除的IC50值分别为7.96 μg/mL、7.22 μg/mL、0.433 μg/mL。


Optimization of extraction technology and antioxidant activity study of tea polyphenols in sun-dried green tea from Fengqing large-leaved species in Yunnan Province
DUAN Fengmin1, ZHANG Shaowang1, SHAO Weijiong2, GAO Hualei2, KUANG Xinggui2, RAO Jie1, BAO Zhijuan2
1. Yunnan Institute of Measuring and Testing Technology, Kunming 650228, China;
2. College of Tobacco Science, Yunnan Agricultural University, Kunming 650201, China
Abstract: Using sun-dried green tea from Fengqing large-leaved species in Yunnan Province as raw material, a Box-Behnken design and response surface analysis was applied to study the effects of ethanol concentration, liquid material ratio, extractive temperature and extractive time on the extraction rate of tea polyphenols based on the single factor experiments. And the antioxidant activity of tea polyphenols were evaluated using the scavenging activity superoxide anion (O2-), ·OH and DPPH· free radical ability. The results showed that when the ultrasonic power was set at 160 W with 0.2 g tea powder, the predictable optimum ultrasonic extraction conditions for tea polyphenols were as follows:ethanol concentration 60.01%, liquid/solid ratio of 49.28:1 mL/g, ultrasonic time 25 min, and ultrasonic temperature 30℃. Under this optimum condition, the total extraction yield was 19.905% (the tested value is 19.83%). The tea polyphenols of sun-dried green tea from Fengqing large-leaved species had an obvious antioxidant activity, the IC50 value of superoxide anion, ·OH and DPPH free radical scavenging ability was 7.96 μg/mL, 7.22 μg/mL and 0.433 μg/mL, respectively.
Keywords: large leaf species;sun-dried green tea;tea polyphenols;response surface analysis;antioxidant activity
2019, 45(5):66-73  收稿日期: 2018-12-14;收到修改稿日期: 2019-01-25
基金项目: 国家质检总局科技计划项目(2017QK191);云南农业大学大学生科技创新创业训练计划项目(2018-180)
作者简介: 段凤敏(1978-),少女,云南凤庆县人,高级工程师,硕士,从事化学计量及尺度物质研究
参考文献
[1] 谢志英, 黄立文, 王秀华, 等. 云南大叶种茶不同品种儿茶素组分含量分析[J]. 中国农学通报, 2014, 30(19):146-150
[2] 杨方慧, 杨毅坚, 张艳梅, 等. 大叶种茶功能成分研究及提取开发现状[J]. 安徽农业科学, 2018, 46(11):10-13
[3] 杨皓彬, 杨娜, 柏雪, 等. 白茶中茶多酚提取工艺及抗氧化活性的研究[J]. 中国食品学报, 2014, 14(12):24-31
[4] 高海荣, 赵爱娟, 陈秀丽, 等. 从茶叶中提取茶多酚工艺的对照研究[J]. 中国食品添加剂, 2017(3):133-137
[5] NIHAL T, FERDA S, Y. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods[J]. Food Chemistry, 2006, 99(4):835-841
[6] 黄明军, 杨新河, 覃彩芹, 等. 响应曲面法优化青砖茶茶多酚的提取工艺[J]. 湖北工程学院学报, 2015, 35(06):5-10
[7] 李洋, 熊道陵, 张建平, 等. 响应面法优化微波-超声耦合双水相提取茶多酚工艺[J]. 江西理工大学学报, 2018, 39(01):52-59
[8] 陈钢, 李栋林, 史建鑫, 等. 响应面试验优化超声耦合双水相体系提取茶多酚工艺[J]. 食品科学, 2016, 37(06):95-100
[9] 唐淯桓, 梁青, 邹佳佳, 等. 响应面法优化红茶茶多酚清除DPPH能力的研究[J]. 茶叶通讯, 2013, 40(01):3-6
[10] 龚文琼, 刘睿. 响应面法优化微波赞助提取普洱茶中茶色素工艺研究[J]. 食品科学, 2010, 31(08):137-142
[11] 王鑫瑶, 赵鑫淼. 茶多酚的提取技术及优缺点比较研究进展[J]. 广州化工, 2018, 46(8):26-27
[12] 保志娟, 方云山, 丁中涛, 等. 分光光度法研究芦丁一铜配合物及其自由基清除活性[J]. 光谱实验室, 2005, 22(2):253-256
[13] MAGDALENA J S, MAGDALENA K, AGNIESZKA Z G. Determination of antioxidant activity, rutin, quercetin, phenolic acids and trace elements in tea infusions:Influence of citric acid addition on extraction of metals[J]. Journal of Food Composition and Analysis, 2015, 40:70-77
[14] ANNA P, KRYSTYNA P. Effect of pH and metal ions on DPPH radical scavenging activity of tea[J]. International Journal of Food Sciences and Nutrition, 2015, 66(1):58-62